Tag: ag-tech

Israel joins the fruit-fly fight

Biofeed’s Nimrod Israely, top centre, with mango growers in Karnataka, India. (photo from Biofeed via Israel21c)

Shortly before Indian Prime Minister Narendra Modi’s visit to Israel in early July, Indian diplomats in Israel heard about a revolutionary no-spray, environmentally friendly solution against the Oriental fruit fly (Bactrocera dorsalis) made by Biofeed, a 10-employee ag-tech company. They invited Biofeed to be one of six innovative Israeli companies meeting with Modi and Israeli Prime Minister Binyamin Netanyahu.

The company’s founder and chief executive officer, Nimrod Israely, who has a PhD in fruit-fly ecology, told the two leaders that Biofeed’s product can protect Indian farmers against fruit flies like the Iron Dome system protects the people of Israel against missiles. The Oriental fruit fly has been decimating 300 fruit species in India and in 65 other countries in Asia, Africa and the Americas and is considered to be the most destructive, invasive and widespread of all fruit flies.

Biofeed’s lures, hung on trees, contain an organic customized mix of food, feeding stimulants and control or therapeutic agents delivered by a patented gravity-controlled fluid release platform. Attracted by the odour, the fly takes a sip and soon dies – without any chemicals reaching the fruit, air or soil.

The launch of Biofeed’s first-in-class attractant for female Oriental fruit flies results from 15 years of development of the core platform and more than a year of development and testing in Israel and Karnataka, India. Mango farmers on four Indian orchards saw an overall decrease of fruit-fly infestation from 95% to less than five percent.

“We were hoping to bring a solution that will replace spraying and increase productivity by 50%,” Israely told Israel21c. “I am excited by the results, demonstrating the future potential for some farmers to bring about 900 times more marketable produce to market.”

photo - A fruit fly feeding in a Biofeed lure
A fruit fly feeding in a Biofeed lure. (photo from Biofeed via Israel21c)

One farmer in the Biofeed pilot explained that previously he had used a trap that attracted only male fruit flies, with limited success. “If you cut 25 fruits, we were getting only one good fruit; 24 were infected,” he said.

K. Srinivas Gowda, president of the 70,000-farmer Karnataka Mango Growers Association, wrote in a letter presented to Modi and Netanyahu that he “would like to have this [Biofeed] technology implemented to all the mango farmers through the government of India. This technology can be used to develop pest-free zones in the mango-growing belts in India.”

The pilot project started after Biofeed won a Grand Challenges Israel grant last year from the Israel Innovation Authority and the Foreign Ministry’s international development agency, Mashav.

“We don’t have the Oriental fruit fly in Israel. However, until now there was no solution for this problem. So, we took the challenge and chose to focus on India,” Israely said. The company worked with Kempmann Bioorganics in Bangalore to carry out the trial.

Biofeed’s products are used in many Israeli fruit orchards against the Mediterranean fruit fly and other common pests, including the olive fruit fly and the peach fruit fly (Bactrocera zonata).

“Bactrocera zonata is the number two pest in India. There are three main pests in India, so now we’ve given, within two years, a solution for the two most devastating fruit flies in India and in other parts of the world,” said Israely.

“We are the only company in the world with a solution for those two pests and both solutions are harmless to the environment,” he added. “We estimate the annual market potential of these two pest segments to be well over $1 billion.”

The Biofeed platform is effective with as few as 10 units per hectare and for a period of nearly a year before the dispenser needs replacing.

Biofeed, founded in 2005, also has a formula targeting mosquitoes that bear viruses such as Zika.

“Evolution has given insects an elaborate sense of smell, which they utilize to find mates, food, egg-laying sites and more,” Israely told Israel21c last year. “The company has developed a liquid formula that ‘knows’ how to tie different kinds of smells to other materials, as the need arises. The result is a special ‘decoy’ that draws the target insect through smell. The decoy is slow-released from a device over the course of a year. The insect is drawn to the decoy, feeds off it and dies shortly after.”

Headquartered in Kfar Truman, Biofeed sees the future of agriculture in developing countries such as India and China.

“We want to bring something that is extremely easy to use: you don’t need tractors, you don’t need to remember to spray once a week, you don’t need to put yourself in danger with sprays, there’s no safety equipment. This is something that can make a dramatic change in agriculture and human health,” said Israely.

For more information, visit biofeed.co.il/enhome.

Israel21c is a nonprofit educational foundation with a mission to focus media and public attention on the 21st-century Israel that exists beyond the conflict. For more, or to donate, visit israel21c.org.

Betalains boost resistance?

Unripe (top) and ripe (bottom) tomatoes. Regular tomatoes (far left) start out green (far left top) and turn red when ripe (far left bottom). In contrast, genetically engineered tomatoes assume different shades of red-violet, depending on whether they produce betalains (the column second from left), pigments called anthocyanins (second from right) or betalains together with anthocyanins (far right). (photo from wis-wander.weizmann.ac.il)

Colour in the plant kingdom is not merely a joy to the eye. Coloured pigments attract pollinating insects, they protect plants against disease, and they confer health benefits and are used in the food and drug industries. A new study conducted at the Weizmann Institute of Science, published in Proceedings of the National Academy of Sciences, USA, has opened the way to numerous potential uses of betalains, the highly nutritious red-violet and yellow pigments known for their antioxidant properties and commonly used as food dyes.

Betalains are made by cactus fruit, flowers such as bougainvillea and certain edible plants – most notably, beets. They are relatively rare in nature, compared to the two other major groups of plant pigments and, until recently, their synthesis in plants was poorly understood. Prof. Asaph Aharoni of Weizmann’s plant and environmental sciences department and Dr. Guy Polturak, then a research student, along with other team members, used two betalain-producing plants – red beet (Beta vulgaris) and four o’clock flowers (Mirabilis jalapa) – in their analysis. Using next-generation RNA sequencing and other advanced technologies, the researchers identified a previously unknown gene involved in betalain synthesis and revealed which biochemical reactions plants use to convert the amino acid tyrosine into betalains.

To test their findings they genetically engineered yeast to produce betalains. They then tackled the ultimate challenge: reproducing betalain synthesis in edible plants that do not normally make these pigments.

photo - Tomatoes that have been genetically engineered to produce betalains only in the fruit, but not elsewhere in the plant
Tomatoes that have been genetically engineered to produce betalains only in the fruit, but not elsewhere in the plant. (photo from wis-wander.weizmann.ac.il)

The success announced itself in living colour. The researchers produced potatoes, tomatoes and eggplants with red-violet flesh and skin. They also managed to control the exact location of betalain production by, for example, causing the pigment to be made only in the fruit of the tomato plant but not in the leaves or stem.

Using the same approach, the scientists caused white petunias to produce pale violet flowers, and tobacco plants to flower in hues varying from yellow to orange pink. They were able to achieve a desired hue by causing the relevant genes to be expressed in different combinations during the course of betalain synthesis. These findings may be used to create ornamental plants with colours that can be altered on demand.

But a change in colour was not the only outcome. Healthy antioxidant activity was 60% higher in betalain-producing tomatoes than in average ones. “Our findings may in the future be used to fortify a wide variety of crops with betalains in order to increase their nutritional value,” said Aharoni.

An additional benefit is that the researchers discovered that betalains protect plants against grey mold, Botrytis cinerea, which annually causes losses of agricultural crops worth billions of dollars. The study showed that resistance to grey mold rose by a whopping 90% in plants engineered to make betalains.

The scientists produced versions of betalain that do not exist in nature. “Some of these new pigments may potentially prove more stable than the naturally occurring betalains,” said Polturak. “This can be of major significance in the food industry, which makes extensive use of betalains as natural food dyes, for example, in strawberry yogurts.”

Furthermore, the findings of the study may be used by the drug industry. When plants start manufacturing betalains, the first step is conversion of tyrosine into an intermediate product, the chemical called L-dopa. Not only is this chemical itself used as a drug, it also serves as a starting material in the manufacture of additional drugs, particularly opiates such as morphine. Plants and microbes engineered to convert tyrosine into L-dopa may, therefore, serve as a source of this valuable material.

The research team included Noam Grossman, Dr. Yonghui Dong, Margarita Pliner and Dr. Ilana Rogachev of Weizmann’s plant and environmental sciences department, and Dr. Maggie Levy, Dr. David Vela-Corcia and Adi Nudel of the Hebrew University of Jerusalem. Aharoni’s research is supported by the John and Vera Schwartz Centre for Metabolomics, which he heads; the Leona M. and Harry B. Helmsley Charitable Trust; the Adelis Foundation; the Lerner Family Plant Science Research Fund; the Monroe and Marjorie Burk Fund for Alternative Energy Studies; the Sheri and David E. Stone Fund for Microbiota Research; Dana and Yossie Hollander, Israel; the AMN Fund for the Promotion of Science, Culture and Arts in Israel; and the Tom and Sondra Rykoff Family Foundation. Aharoni is the recipient of the André Deloro Prize, and the incumbent of the Peter J. Cohn Professorial Chair.

For more on the research being conducted at the Weizmann Institute, visit wis-wander.weizmann.ac.il.